ABSTRACT
Objective To investigate the role of FTY720 in the protection of rat renal ischemia reperfusion (I/R) injury and its influence on the expression of hypoxia inducible factor-1? (HIF-1?).Methods Ischemia/reperfusion kidney injury rat model was established. The rats were allocated into groups at random: sham group (Sham) and ischemia/reperfusion group (Control). The latter were sub-divided into FTY720 (1 mg/kg every day for 3 days before operation) group, MMF (20 mg/kg every day for 3 days before operation) group and CsA (10 mg/kg every day for 3 days before operation) group. Blood creatinine and the expression levels of HIF-1? mRNA and protein were observed 0 h, 6 h, 24 h, 72 h and 7 days after operation.Results At time points of 0 h , 6 h , and 24 h, Scr levels were higher in FTY720 group than in control group (P
ABSTRACT
Objective To explore the inhibitory effect of mycophenolic acid (MPA) on the proliferation and monocyte chemoattractant protein-1 (MCP-1) secretion in mice glomerular mesangial cells (GMCs).Methods MPA with different concentrations ( 0.1 , 1, 5 and 10 ?mol/L ) was added into the cultured mice GMC line in vitro for 24 h. The GMCs proliferation level was detected by the 3-(4,5-Dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) incorporation. The absorbance for counting the GMCs survival was read at 570 nm by using an automated microplate ELISA reader. The cells treated with MPA (0, 0.5 and 2.5 ?mol/L ) were stimulated by TNF-? (20 ng/ml), IL-1? (2 ng/ml), IFN-? (10 ng/ml). Four h later, the MCP-1 concentration in supernatant was determined by ELISA. At the same time, the lymphocyte migration was examined.Results MPA has a concentration -dependent antiproliferative effect on cultured GMCs. The secretion of MCP-1 of cultured GMCs stimulated by TNF-?, IL-1?, IFN-? was significantly increased. Addition of MPA could signi -ficantly inhibit the secretion of MCP-1 ( P